Polarographic Studies on Enzymes

Polarographic Studies of Human Blood Sera

R. BrdiEka, in 1937, in a note to the editor of Nature (1), reported the application of the polarographic method to cancer diagnosis, through the measurement of certain sulphur-containing amino acids present in the proteins in human blood serum. Brdicka made tests on the serum from 67 different persons; 32 of these gave an abnormally low polarographic protein effect. These 32 cases were shown b...

Studies on Bacterial Enzymes

The meningococcus cell contains a peptonase enzyme which hydrolyzes the peptides and similar constituents of commercial "peptone." The activity of this enzyme is independent of the presence of the formed bacterial cell. The peptonase enzyme is more resistant to heat and to oxidation than is the maltase enzyme of the same bacteria.

Studies on the Enzymes

In the preceding paper (1) are recorded the facts so far obtained in a study of the proteolytic enzymes of pneumococcus. It has been shown that bile solutions of pneumococci, extracts obtained by disintegration of the organisms in phosphate mixtures, and sterile filtrates of autolyzing broth cultures possess the power to hydrolyze peptones and to a less extent certain intact proteins. In the pr...

Studies on Thf, Enzymes of Pneumococcus

Studies on the Enzymes of Pneumococcus

1. Pneumococci contain an intracellular enzyme of marked lipolytic activity as measured by the acid liberated by its action on tributyrin. 2. Enzyme-containing solutions may be prepared by dissolving pneumococci in bile, or by extraction by other means. 3. The optimum reaction for maximum activity of the endolipase is about pH 7.8, which coincides with the optimum hydrogen ion concentration for...